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2.1 The webinars

The two webinars were organised during the COVID-19 pandemic in 2021.

Webinar 1: "Why gene banking?"

The first webinar (23.6.2021) had six invited speakers:
Mervi Honkatukia: The current status of Nordic gene banking. A case report in IMAGE EU Horizon 2020.
Annika Tienhaara: Benefits of conserving agricultural genetic resources in Finland: Summary of recent Finnish research and setting it in the international context.
Sipke Joost Hiemstra: Introduction on gene bank activities in Europe.
Mike Bruford: The Frozen Ark: Saving the DNA and viable cells of the world’s endangered species.
Sigbjørn Gregusson: Introduction of Biobank AS.
Per Johan: How to secure/develop the original Nordic red breeds?
There were 39 registered online participants from six countries (Figure 1 and Table 1). Following the presentations the participants discussed in groups about contemporary topics, including:
  1. Outreach activities - informing and educating the public (and students to engage the next generation of experts?) of the importance of genetic resource banking,
  2. Current technological limitations (bottlenecks) in the cryopreservation of animal germplasm – future research priorities, and
  3. Practical solutions to strengthen conservation.
Figure 1: The country wise distribution of the participants for webinar 1. Date: 23.6.2021. Number of registered participants: 39.
Participating organisations and universities:
Häme University of Applied Sciences (HAMK)
Norwegian Institute of Bioeconomy Research (NIBIO)
Norwegian Genetic Resource Centre
Nordic Genetic Resource Center (NordGen)
Natural Resources Institute Finland (Luke)
SEGES Innovation
Norwegian University of Life Sciences (NMBU)
Norwegian Association of Sheep and Goat Breeders (NSG)
Norsk Rikstoto
Swedish University of Agriculture (SLU)
Norwegian Horse Association (NHS)
NORSVIN
Viking Genetics
The Department of Animal and Veterinary Sciences – ANIS health, Aarhus University
Landbrugsbestyrelsen
Finnish Animal Breeding Association (FABA)
Lammasmaailma
The Faculty of Health Sciences, University of Copenhagen
ProAgria
The Agricultural Agency of Faroe Islands
Norsk Fjordhestsenter (Norwegian association for fjord horse)
BioBank AS
Table 1: Participating organisations and universities in webinar 1. Date 23.6.2021. Number of registered participants: 39. Number of participating organisations and universities: 23.
Conclusions from the group discussion can be divided into the previously listed topics. The general census regarding the first topic comprised the necessity of education regarding genetic diversity and conservation, the importance of collaboration, and integrated multidisciplinary efforts towards ecosystem services and reinvention of local products. Education and open discussions surrounding genetic diversity, conservation and new technologies was encouraged for different audiences (i.e., schools, farmers, consumers, scientists, administrators, funding agencies and the public) by appropriate channels for each of the target audiences (e.g., social media, books, publications, seminars, exhibitions etc.).
For the second topic logistical and technical limitations associated with sampling of small and isolated populations were identified. Furthermore, it was noted that most of the ex-situ gene bank specimens are semen samples. For successful management of small populations and for the use of assisted reproductive technologies (ARTs) conservation of female germline is also crucial. However, species-specific differences of female germlines require further research for the optimized cryopreservation protocols free of animal-derived compounds and antibiotics. Participants highlighted that an evaluation of the already cryopreserved material in the Nordic countries would be valuable, as this would provide an overview of material that could be used as the basis for possible collaborative complementary cryoconservation in the region. The importance and feasibility of having geographically distributed back-up gene banks was discussed. The costs, legislation and regulations for transportation of samples that should be defined were considered. An educative workshop on FAO’s cryoconservation protocols was suggested.
Lastly, the third topic had the following conclusions: There is a need to harmonize/standardize high-biosecurity protocols for the collection and storage of selected tissues. Species-specific differences in germlines require further research for the optimized cryopreservation protocols. Furthermore, the possibilities for financing gene banks were also discussed as an important factor for strengthening conservation efforts.

Webinar 2: “Arvokas geeniperimä pakkaseen – Eläin- ja kasvigeenivarojen pankittamisen nykytilanne ja haasteet”

(Cryoconservation of the valuable genetic resources – The current situation and challenges of gene banking animal and plant genetic resources)

The second webinar (8.12.2021) had five invited speakers:
Jaana Peippo: "NordFrost-verkoston esittely." (Eng: Introduction to the NordFrost network.)
Pekka Uimari: "Pienten populaatioiden jalostamisen ja säilyttämisen haasteet." (Eng: Challenges of management and conservation of small populations.)
Heli Lindeberg: "Eläingeenipankkitoiminnan mahdollisuudet ja haasteet." (Eng: Possibilities and challenges of gene banking of animal genetic resources.)
Anna Nukari: "Kasvigeenipankkitoiminnan mahdollisuudet ja haasteet." (Eng: Possibilities and challenges of gene banking of plant genetic resources.)
Tuula Pehu: "Geenivarojen hallinta." (Eng: Ownership of genetic resources.)
There were 59 registered online participants from Finland (Figure 2, Table 2). Before the presentations the participants discussed in groups, about the following questions:  
  1. Onko Suomen kansallinen geenivaraohjelma sinulle tuttu, tunnetko sen sisällön? (Eng: Are you familiar with the Finnish National Genetic Resources Programme for Agriculture, Forestry and Fishery, do you know its content?)
  2. Minullako arvokkaita geenivaroja? (Eng: Do I have valuable genetic resources?)
  3. Miten mielestäni yhteydenpito tuottajien ja geenivarakoordinaattorin välillä pitäisi toteuttaa? (Eng: How do I think communication between producers and the national coordinator should be implemented?).
  4. Kuka omistaa / kenen pitäisi omistaa geenivarat? (Eng: Who owns / who should own the genetic resources?).
  5. Geenipankki ja eläintautien torjunta, miten lisäisit geenivarojen tunnettuutta – alkutuottajien tekemän geenivarasäilytyksen statuksen parantaminen? (Eng: The gene bank and the fight against animal diseases: How would you increase the awareness of genetic resources – improving the status of genetic resource preservation by primary producers?)
  6. Geenivarojen säilytyksen taloudelliset näkökulmat, Miten näet geenivarojen tulevaisuuden? Terveiset geenipankille! (Eng: Economic aspects of conserving genetic resources: How do you see the future of genetic resources? Greetings to gene bank!)
Figure 2: Background of participants in webinar 2. Date 8.12.2021. Number of registered participants: 59.
Participating organisations and universities:
Finnish Animal Breeding Association (FABA)
Oulu University of Applied Sciences (OAMK)
Häme University of Applied Sciences (HAMK)
Savonia University of Applied Sciences (Savonia UAS)
Natural Resources Institute Finland (Luke)
Ministry of Agriculture and Forestry (MMM)
Ministry of Education (OM)
Viking Genetics
Maaseudun tulevaisuus
Suomen Hippos ry
ProAgria
Lammasmaailma
Nordic Genetic Resource Center (NordGen)
Suomen siemenperunakeskus Oy
Kainuun ammattiopisto (KAO)
Suomen Kennelliitto
Table 2: List of organisations and universities that the participants were representing. Date 8.12.2021.
For the group discussions participants were divided in five groups: cattle, horses, bees, plants and mixed. The groups used the given questions as a basis for their discussions, but with some modifications due to the limited time and interest of the participants. Conclusions from the discussions can be summarised as follows:
Cattle
  1. The knowledge about the content of The Finnish National Genetic Resources Programme for Agriculture, Forestry and Fishery is superficial and depends on the farmers’ own activity.
  2. More science-based knowledge is needed for the different genetic lines within the native cattle breeds. Farmers consider that all production systems should be valued, e.g. both dairy and suckler cow production. Also, according to the participants, the concept of unique genetic resources should not be limited only to registered individuals. “It is time to focus on quality instead of quantity.” 
  3. Up-to-date communication between the different stakeholders is considered very important. Farmers also hope for the establishment of a steering group for the management of the native cattle genetic resources. Farmers lack accurate science-based information about the conservation programme. It is not considered to be enough to have just pedigree and traditional knowledge-based information. Regular up-to-date reporting is also considered important. Farmers hope for an updated database to aid their decision making in breeding. At the same time farmers expressed concerns about bureaucratic involvement in a conservation programme.
  4. Sample collection into a gene bank is based on mutual agreement between the animal owner and the gene bank.
Horse
  1. The conservation programme of the Finnhorse is not well known. Horse owners hope for more publicly available information.
  2. Public knowledge about the composition of the gene bank working group is missing, who are the members?
  3. Public knowledge should also be available about the selection criteria of the Finnhorse stallions collected for the gene bank.
  4. Using ARTs (cryopreservation of semen/ sperm and oocytes, ICSI) in horses is considered expensive.
  5. Collaboration with the commercial equine sector is considered to benefit the conservation programme in the long run.
  6. Somatic cell cloning may be used as tool to preserve native horse breeds.
Honeybee
  1. Beekeeping of the Nordic native brown bee is not secure in Finland, because the population size of this subspecies is small, and only a small number of bee farmers are interested in keeping these bees.
  2. Only semen cryopreservation is successful now. Successful cryopreservation of eggs would enable desired breeding goals.
  3. Exchange of information is easy due to the small group of bee farmers but requires personal relationships and own activity. It requires constant activity to have enough publicity to succeed in the conservation of the Nordic brown bee.
  4. Bee farmers own their own genetic resources.
  5. Most viruses may be spread through inseminations and affect the breeding success of queens. It would be beneficial to have a diagnostic tool to assess sperm quality/ sanitary status.
  6. In the future, there will be costs for testing the purity of the Nordic brown bees from DNA to ensure conservation of the brown bee genetic resources.
  7. Cryoconservation is not yet optimised in bees. It is therefore necessary to follow international research.
Mixed group
  1. Different animal groups need to communicate/ be informed about their contemporary conservation status (future aims should be better known)
  2. The public should be informed about the conservation programmes of our versatile animal genetic resources, including the content of the programmes and the parties involved.
  3. The situation for many breeds/ subspecies is critical as circumstances change rapidly and genetic diversity is lost in every generation.
  4. It would be beneficial for the conservation of the genetic resources of chicken if cryopreserved semen samples could be used successfully.
  5. It was discussed what is reasonable compensation for the contribution in conservation of genetic diversity.
  6. It is important to inform the public and/ or decision-makers that disease status changes rapidly, and that ex-situ gene banking is one solution that helps us to be prepared for the circumstances where a disease outbreak has caused extinction of an entire breed, or genetic diversity must be re-introduced into a population suffering from inbreeding depression.
  7. The maedi-visna programme for sheep farms is needed. At the moment it is voluntary, and sheep farmers resign from it.
Plants
  1. It is crucial to improve communication so that information is more easily available for the stakeholders.
  2. It would be beneficial to supervise the public on how to recognize and classify valuable genetic resources.
  3. It is also beneficial for the stakeholders to know what already exists in gene banks. NordGen maintains seeds for all the Nordic countries and its collections include around two thousand accessions of the Finnish origin. Around 1000 accessions of clonally propagated species varieties are conserved in the Finnish clonal gene banks in the field collections. The data that has been stored in of the national clonal collections will also be uploaded to the NordGen’s database, where it will be publicly available, but it may be laborious to find. Most data are uploaded already.
  4. GENBIS could serve as an important platform for this communication. It is worth informing the public where it can be found and how the plant data can be ordered.
  5. There is a wish for more transparency and collaboration for the plant gene banks. The participants feel this will improve knowledge-sharing and important stakeholders to openly discuss which actions that are desired and needed from the genetic resources in the gene banks.
  6. Luke maintains the gene bank of the clonally propagated plant species, and its accessions are safeguarded by cryopreservation (cryotanks in two locations) and by the safety maintainers network (outside Luke).
  7. Genetic resources of cultivated plants should be available for plant breeding to ensure future food security. Through the gene banks they are available by SMTA (standard material transfer agreement) according to the International Treaty.
  8. Cryoconservation offers a good approach to avoid plant diseases. Cryopreserved disease-free materials can be used to revitalize and renew gene bank field collections or commercial nurseries used for reproduction and marketing.
  9. There could be interested customers for “cryoservice” since the establishment of cryoconservation is expensive. It is worth exploring whether Luke could sell such a service.
  10. Because cryoconservation is rather expensive, it is crucial to communicate the value and importance of genetic resources to the public.
  11. It is difficult to give financial value to the genetic resources. A gene bank that acts as a backup storage, can be shown to outsiders only as an expense, but it is indeed priceless material.
  12. In Finland, subsidies are available for the cultivation of the registered conservation varieties, as well as for the network that maintains the backup collections of clonally propagated plant genetic resources.
  13. It is of high importance to secure genetic resources and especially species that are under severe threat of diseases to safely maintain them for future use.